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Correction: Brandão et al. Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO<sub>2</sub> NM). <i>Nanomaterials</i> 2021, <i>11</i>, 1502.

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Atopic Dermatitis Studies through In Vitro Models.

Evelyne De Vuyst1, Michel Salmon2, Céline Evrard1

  • 1Cell and Tissue Laboratory, URPhyM-NARILIS, University of Namur, Namur, Belgium.

Frontiers in Medicine
|August 10, 2017
PubMed
Summary

In vitro models are crucial for understanding atopic dermatitis (AD) pathogenesis and developing new treatments. This review details various models, from simple cell cultures to complex tissue equivalents, aiding research and drug screening for AD.

Keywords:
atopic dermatitisepidermal keratinocytesin vitromodelreconstructed epidermisskin equivalents

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Area of Science:

  • Dermatology
  • Immunology
  • In vitro modeling

Background:

  • Atopic dermatitis (AD) is a complex inflammatory skin disease.
  • Epidermal barrier defects and immune dysregulation are key to AD pathogenesis.
  • Understanding AD etiology is vital for developing effective treatments.

Purpose of the Study:

  • To review various in vitro models that mimic features of atopic dermatitis.
  • To provide context for selecting appropriate experimental models for AD research.

Main Methods:

  • Review of 2D cell culture systems (immune cells, keratinocytes).
  • Analysis of 3D skin/epidermal equivalents with barrier properties.
  • Examination of models using cytokine challenges or gene silencing.
  • Inclusion of models with co-cultured lymphocytes or patient cells.

Main Results:

  • In vitro models successfully replicate key features of AD.
  • Different models offer varying levels of complexity and relevance.
  • Models facilitate the study of AD pathogenesis and the screening of therapeutic compounds.

Conclusions:

  • In vitro models are valuable tools for advancing AD research.
  • These models aid in understanding disease mechanisms.
  • They are instrumental in discovering and testing new therapeutic strategies for AD.