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Updated: Feb 25, 2026

A Protocol for Roux-en-Y Gastric Bypass in Rats using Linear Staplers
Published on: August 21, 2021
Laparoscopic Linear Stapled Running Enterotomy Closure in Roux-en-Y Gastric Bypass Using Absorbable Unidirectional
Ben Gys1, Tobie Gys2, Michael Ruyssers2
1AZ Sint-Dimpna, J-.B. Stessensstraat 2,, 2440, Geel, Belgium. ben.gys@gmail.com.
Background:
Laparoscopic running enterotomy closure for linear stapled Roux-en-Y gastric bypass (RYGB) may be enhanced by using unidirectional barbed sutures (Stratafix™ 2/0, Ethicon) as it eliminates the need for knot tying and assistance from a third hand.
Objectives:
The objective of this paper is to present our technique using unidirectional barbed sutures (Stratafix™ 2/0, Ethicon).
Methods:
After stapling the gastrojejunostomy, we start the closure of the residual enterotomy unidirectional from left to right (single, full-thickness layer) which is cut without a knot. For the jejunojejunostomy, the residual enterotomy is closed perpendicular from top to bottom in order to avoid iatrogenic stricture formation (single, full-thickness layer). No backstitches are performed.
Conclusions:
We feel strongly that this technique might enhance running enterotomy closure for linear stapled RYGB.

