Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.4K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.4K
Bone Cells and Tissue01:30

Bone Cells and Tissue

8.8K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
8.8K
Bone as Supporting Connective Tissue01:23

Bone as Supporting Connective Tissue

7.5K
Bone tissue forms the internal skeleton of vertebrate animals, providing structure to the body.
Bone Matrix
Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts—...
7.5K
The Functions of the Skeletal System01:22

The Functions of the Skeletal System

6.2K
The most apparent functions of the skeletal system are support, protection, and movement. However, bone tissue also performs several other critical metabolic functions. For one, the bone matrix acts as a reservoir for a number of minerals important to the functioning of the body, especially calcium and phosphorus. These minerals, present in the bone tissue, can be released back into the bloodstream when required. Calcium ions, for example, are essential for muscle contractions and controlling...
6.2K
Membrane Fluidity01:26

Membrane Fluidity

17.1K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
17.1K
The Bone Matrix01:18

The Bone Matrix

6.0K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondria as a Therapeutic Target for Burn Injury.

Biomolecules·2026
Same author

Impaired mitochondrial stress signaling mediates bone loss in male mice in the absence of BNIP3.

bioRxiv : the preprint server for biology·2026
Same author

Dietary effects on cardiac lipid composition, mitochondrial respiration, stress proteins and thermal tolerance in the American lobster (Homarus americanus).

The Journal of experimental biology·2025
Same author

Morphometric vertebral fractures at hospitalization associate with Long COVID occurrence.

Journal of endocrinological investigation·2025
Same author

Canagliflozin-Induced Adaptive Metabolism in Bone.

Diabetes·2025
Same author

Nuclear Condensates of WW Domain-Containing Adaptor With Coiled-Coil Regulate Mitophagy via Alternative Splicing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same journal

When LXA4 meets white fat: browning via the miR-133a-3p/SIRT1 pathway in mouse models.

Adipocyte·2026
Same journal

Twf1 regulates adipocyte proliferation and differentiation through Yap nucleus accumulation.

Adipocyte·2026
Same journal

Normalized periprostatic adipose tissue thickness: an imaging marker associated with prostate biopsy outcomes among patients with PI-RADS and PSA double gray zone.

Adipocyte·2026
Same journal

Multi-omics integration reveals convergent extracellular matrix remodelling and lipid metabolic reprogramming as central axes of adipocyte differentiation from mouse embryonic stem cells.

Adipocyte·2026
Same journal

Wt1 facilitates visceral beige fat formation to combat abdominal obesity.

Adipocyte·2026
Same journal

A non-invasive method for light-inducible knockout across all cell types in mouse subcutaneous adipose tissue.

Adipocyte·2026
See all related articles

Related Experiment Video

Updated: Feb 25, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K

Intracellular lipid droplets support osteoblast function.

Elizabeth Rendina-Ruedy1, Anyonya R Guntur1, Clifford J Rosen1

  • 1a Center for Clinical and Translational Research , Maine Medical Center Research Institute , Scarborough , ME , USA.

Adipocyte
|August 10, 2017
PubMed
Summary
This summary is machine-generated.

Osteoblasts, the bone-building cells, accumulate lipid droplets during bone formation. These droplets supply fatty acids, crucial energy substrates for osteoblast differentiation and increased bone mass.

Keywords:
bonefatty acidslipolysislipophagymarrow adipocytes

More Related Videos

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

20.5K
Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
05:37

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Published on: December 28, 2019

12.7K

Related Experiment Videos

Last Updated: Feb 25, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

20.5K
Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
05:37

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Published on: December 28, 2019

12.7K

Area of Science:

  • Cell Biology
  • Metabolism
  • Bone Biology

Background:

  • Bone formation requires significant energy for matrix protein secretion and mineralization.
  • Glucose is considered the primary energy source for osteoblasts, but fatty acid utilization is increasingly recognized.
  • Lipid droplet accumulation, common in many cell types, has not been well-documented in osteoblasts.

Purpose of the Study:

  • To investigate whether osteoblastogenesis is associated with lipid droplet accumulation.
  • To determine if osteoblasts can utilize accumulated lipid droplets as an energy source.
  • To explore the role of fatty acids in osteoblast metabolic programming for bone formation.

Main Methods:

  • Cell culture of osteoblasts.
  • Microscopic analysis to detect lipid droplets.
  • Biochemical assays to assess energy substrate utilization during differentiation.

Main Results:

  • Osteoblast differentiation was accompanied by a significant increase in intracellular lipid droplet accumulation.
  • These lipid droplets served as a source of fatty acids, providing energy substrates for osteoblastogenesis.
  • Evidence suggests osteoblasts can metabolically adapt to utilize fatty acids during bone formation.

Conclusions:

  • Osteoblastogenesis involves lipid droplet accumulation, highlighting a previously underappreciated metabolic pathway.
  • Fatty acids derived from lipid droplets are essential energy substrates supporting osteoblast differentiation.
  • Targeting fatty acid metabolism in osteoblasts may offer novel therapeutic strategies for enhancing bone formation and treating bone diseases.