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Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

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An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
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Related Experiment Video

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Isolation, Processing and Analysis of Murine Gingival Cells
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Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

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Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response.

David-Nicolas Morand1, Olivier Huck2,3, Laetitia Keller4

  • 1INSERM (French National Institute of Health and Medical Research), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine laboratory, Faculté de Médecine, FMTS, Strasbourg Cedex F-67085, France. davidnicolas.morand@gmail.com.

Materials (Basel, Switzerland)
|August 11, 2017
PubMed
Summary

Functionalized nanofibrous membranes enhanced the anti-inflammatory effects of alpha-melanocyte stimulating hormone (α-MSH) on gingival cells. This biomaterial approach reduced inflammatory markers and cell proliferation in response to Porphyromonas gingivalis lipopolysaccharide.

Keywords:
anti-inflammatory agentscytokinesepithelial cellsfibroblastslipopolysaccharidespolycaprolactone

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Area of Science:

  • Biomaterials Science
  • Oral Biology
  • Inflammation Research

Background:

  • Alpha-melanocyte stimulating hormone (α-MSH) possesses anti-inflammatory properties beneficial for wound healing.
  • Polyelectrolyte layers enhance α-MSH's anti-inflammatory efficacy when associated with various supports.
  • Gingival inflammation is a key factor in periodontal diseases, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To investigate the impact of nanofibrous membranes functionalized with α-MSH linked to polyelectrolyte layers on gingival cell inflammatory responses.
  • To evaluate the efficacy of Poly-L-glutamic acid conjugated with α-MSH (PGA-α-MSH) on Poly-ε-caprolactone (PCL) membranes.
  • To assess the modulation of inflammatory markers and cell behavior in human oral epithelial cells (EC) and fibroblasts (FB).

Main Methods:

  • Human EC and FB cultured on plastic or electrospun PCL membranes functionalized with PGA-α-MSH.
  • Cells stimulated with Porphyromonas gingivalis lipopolysaccharide (Pg-LPS).
  • Assessed cell proliferation (AlamarBlue), migration (scratch assay), and inflammatory gene expression (IL-6, TNF-α, TGF-β) via RT-qPCR.

Main Results:

  • On plastic, PGA-α-MSH reduced EC and FB migration and decreased IL-6 and TGF-β expression in Pg-LPS stimulated EC.
  • PGA-α-MSH functionalized PCL membranes significantly reduced proliferation of Pg-LPS stimulated EC and FB.
  • A notable decrease in IL-6, TNF-α, and TGF-β expression was observed in Pg-LPS stimulated EC and FB cultured on functionalized membranes.

Conclusions:

  • Functionalization of nanofibrous PCL membranes with PGA-α-MSH effectively amplifies the anti-inflammatory properties of α-MSH.
  • This biomaterial strategy shows promise for mitigating inflammatory responses in gingival cells.
  • The developed material could be a potential therapeutic approach for inflammatory conditions in the oral cavity.