Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Activation of Integrins

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Integrins

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...

Adherens Junctions

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Selective degradation of platelet BTK by PROTAC NX-5948 provides antithrombotic benefits without affecting haemostasis.

Blood advances·2026

Same author

IL-6 as a Mediator of Platelet Hyper-Responsiveness.

Cells·2025

Same author

Difluorinated thromboxane A<sub>2</sub> reveals crosstalk between platelet activatory and inhibitory pathways by targeting both the TP and IP receptors.

British journal of pharmacology·2024

Same author

Platelet procoagulant membrane dynamics: a key distinction between thrombosis and hemostasis?

Blood advances·2022

Same author

Chemical degradation of BTK/TEC as a novel approach to inhibit platelet function.

Blood advances·2022

Same author

Higher body mass index raises immature platelet count: potential contribution to obesity-related thrombosis.

Platelets·2022

Search research articles

Related Experiment Video

Updated: Feb 25, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Integrin α_IIbβ₃ outside-in signaling.

Tom N Durrant¹, Marion T van den Bosch¹, Ingeborg Hers¹

¹School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, United Kingdom.

|August 11, 2017

Summary

Integrin α_IIbβ₃, a key platelet receptor, mediates hemostasis and thrombosis. This review details its complex outside-in signaling pathways, crucial for platelet function and clot formation.

More Related Videos

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Published on: August 8, 2019

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

Related Experiment Videos

Last Updated: Feb 25, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Published on: August 8, 2019

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

Area of Science:

Biochemistry
Cell Biology
Hematology

Background:

Integrin α_IIbβ₃ is a critical platelet receptor involved in hemostasis and thrombosis.
It mediates bidirectional signaling across the plasma membrane, essential for platelet activation and function.

Purpose of the Study:

To provide a comprehensive overview of α_IIbβ₃ outside-in signaling.
To identify key signaling players and their cooperative mechanisms.
To highlight current knowledge gaps and future research directions.

Main Methods:

Literature review of integrin biology and platelet signaling.
Analysis of molecular mechanisms in α_IIbβ₃ outside-in signaling.
Synthesis of information on key signaling components and pathways.

Main Results:

α_IIbβ₃ outside-in signaling is a complex process involving numerous enzymes, adaptors, and cytoskeletal elements.
Ligand binding and integrin clustering trigger downstream signaling cascades.
These cascades amplify cellular events like platelet spreading and clot retraction.

Conclusions:

Understanding α_IIbβ₃ outside-in signaling is vital for comprehending platelet biology.
Further research is needed to fully elucidate the intricate network of signaling components.
This knowledge can inform therapeutic strategies for bleeding and clotting disorders.