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An open-pattern droplet-in-oil planar array for single cell analysis based on sequential inkjet printing technology.

Chenyu Wang, Wenwen Liu, Manqing Tan

  • 1State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, People's Republic of China.

Biomicrofluidics
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A new single-cell analysis method uses inkjet printing to sequentially add reagents to individual cells. This technique efficiently measures intracellular enzyme activity, advancing the study of cellular heterogeneity.

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Area of Science:

  • Cell Biology
  • Biotechnology

Background:

  • Cellular heterogeneity is a key biological principle.
  • A need exists for accessible single-cell research tools to study this heterogeneity.

Purpose of the Study:

  • To develop a novel method for single-cell analysis combining well arrays and inkjet printing.
  • To enable efficient measurement of intracellular activity at the single-cell level.

Main Methods:

  • Cells were captured in cell-sized wells as primary droplets and sealed with oil.
  • Piezoelectric inkjet printing was used to inject lysis buffer and substrate as secondary droplets.
  • Intracellular β-galactosidase activity was measured in K562 cells.

Main Results:

  • High cell capture efficiency (74.5%) was achieved.
  • Successful sequential addition of reagents to single cells was demonstrated.
  • Intracellular β-galactosidase activity was accurately measured at the single-cell level.

Conclusions:

  • The developed method allows simultaneous high single-cell occupancy and sequential reagent addition.
  • This approach offers a flexible and feasible universal tool for single-cell research.
  • The study promotes the use of inkjet printing for investigating cellular heterogeneity.