Related Experiment Video
Updated: Feb 24, 2026
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
The miR-290-295 cluster as multi-faceted players in mouse embryonic stem cells
Kai Yuan1, Wen-Bing Ai2, Lin-Yan Wan1,3
1Institute of Organ Fibrosis and Targeted Drug Delivery, Medical College, China Three Gorges University, 8 Daxue Road, Xiling District, Yichang, 443002 China.
Abstract:
Increasing evidence indicates that embryonic stem cell specific microRNAs (miRNAs) play an essential role in the early development of embryo. Among them, the miR-290-295 cluster is the most highly expressed in the mouse embryonic stem cells and involved in various biological processes. In this paper, we reviewed the research progress of the function of the miR-290-295 cluster in embryonic stem cells. The miR-290-295 cluster is involved in regulating embryonic stem cell pluripotency maintenance, self-renewal, and reprogramming somatic cells to an embryonic stem cell-like state. Moreover, the miR-290-295 cluster has a latent pro-survival function in embryonic stem cells and involved in tumourigenesis and senescence with a great significance. Elucidating the interaction between the miR-290-295 cluster and other modes of gene regulation will provide us new ideas on the biology of pluripotent stem cells. In the near future, the broad prospects of the miRNA cluster will be shown in the stem cell field, such as altering cell identities with high efficiency through the transient introduction of tissue-specific miRNA cluster.
Insights
The miR-290-295 cluster is crucial for embryonic stem cell (ESC) pluripotency, self-renewal, and reprogramming. This microRNA cluster also impacts cell survival, tumor formation, and aging, offering future therapeutic potential in regenerative medicine.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Molecular Biology
Background:
- Embryonic stem cell specific microRNAs (miRNAs) are vital for early embryonic development.
- The miR-290-295 cluster is highly expressed in mouse embryonic stem cells and regulates key biological processes.
Purpose of the Study:
- To review the functional roles of the miR-290-295 cluster in embryonic stem cells.
- To highlight its significance in pluripotency, self-renewal, reprogramming, and cell survival.
- To discuss its implications in tumorigenesis and senescence.
Main Methods:
- Literature review of existing research on the miR-290-295 cluster.
- Analysis of studies investigating miRNA function in embryonic stem cells.
- Synthesis of findings on the cluster's regulatory roles and biological impact.
Main Results:
- The miR-290-295 cluster is essential for maintaining embryonic stem cell pluripotency and self-renewal.
- It plays a role in reprogramming somatic cells into an embryonic stem cell-like state.
- The cluster exhibits a pro-survival function in embryonic stem cells and is implicated in tumorigenesis and senescence.
Conclusions:
- Understanding the miR-290-295 cluster's interactions with gene regulation offers new insights into pluripotent stem cell biology.
- The cluster holds significant promise for future applications in stem cell therapies, including efficient cell identity alteration.
More Related Videos
11:37Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
Published on: November 24, 2015
15:13Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
Published on: April 27, 2017
Related Concept Videos
00:58Embryonic Stem Cells
00:57Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...