Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The application of shear wave elastography in monitoring renal allografts.

Quantitative imaging in medicine and surgery·2026

Same author

Testicular malakoplakia in a middle-aged Han Chinese male: a case report and review of the literature.

Journal of medical case reports·2026

Same author

Glycemic variability does not provide incremental prognostic value for in-hospital death in community-acquired pneumonia patients: conventional clinical variables dominate.

BMC pulmonary medicine·2026

Same author

A Review of the Activity Regulation of Au and Pt Bimetallic Nanozymes and Their Application in Food Safety Analysis.

Biosensors·2026

Same author

Quantification of mitral regurgitation: from traditional methods to artificial intelligence.

Cardiovascular ultrasound·2026

Same author

Atypical presentation of glomus tumor in the facial superficial vein on high-frequency ultrasound: a case report.

Frontiers in medicine·2026

Search research articles

Related Experiment Video

Updated: Feb 24, 2026

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

Microfluidic device for primary tumor spheroid isolation.

Jiaojiao Zhou¹, Jimmy Su², Xiaotong Fu²

¹Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD USA.

Experimental Hematology & Oncology

|August 11, 2017

Summary

This study introduces a microfluidic device for isolating primary tumor spheroids, offering a more accurate model for drug discovery and cancer research than traditional cell cultures.

Keywords:

Cancer Cell Culture Microfluidics Organoid Primary Cell Spheroid

More Related Videos

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

Published on: February 18, 2017

Related Experiment Videos

Last Updated: Feb 24, 2026

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

Published on: February 18, 2017

Area of Science:

Oncology
Biotechnology
Cell Biology

Background:

Two-dimensional (2-D) monolayer cell cultures present significant differences from in vivo physiological conditions, leading to inaccurate data in drug discovery and pathology.
Limitations of 2-D cultures include loss of original in vivo features after passages and altered cellular function due to different morphology.
Three-dimensional (3-D) multi-cellular spheroids better mimic in vivo conditions while retaining in vitro culture advantages, with primary spheroids being ideal for cell-based assays.

Purpose of the Study:

To develop and report a microfluidic device for the efficient isolation of primary tumor spheroids.
To provide a more physiologically relevant model for studying tumor characteristics and drug responses compared to traditional 2-D cell cultures.

Main Methods:

A novel microfluidic device was designed and utilized for the isolation of primary tumor spheroids.
Experiments were conducted using pancreatic tumor samples obtained from mice.

Main Results:

The microfluidic device successfully isolated primary tumor spheroids from pancreatic tumor samples.
Isolated spheroids were maintained in culture for a duration of up to two weeks.

Conclusions:

The developed microfluidic device represents a significant advancement in the isolation of primary tumor spheroids.
This technology has the potential to enhance future drug testing and the interrogation of tumor characteristics.