Label-Free Direct Electrical Detection of a Histidine-Rich Protein with Sub-Femtomolar Sensitivity using an Organic...

There is a growing interest in achieving sensor systems to enable on-site testing of biomarkers. Herein, a new strategy for highly sensitive protein detection at sub-femtomolar levels without any labelling has been demonstrated by using an organic field-effect transistor (OFET). An artificial histidine-rich protein receptor (Ni^II-nitrilotriacetic acid complex, Ni^II-nta) functionalizes a detection portion (i.e. an extended-gate electrode) of the fabricated OFET device. The OFET responds electrically and selectively to a target analyte (bovine serum albumin), meaning that the binding processes at the Ni^II-nta on the extended-gate electrode for the analyte affect the field-effect properties of the device. Our results demonstrate that the combination of the OFET with the artificial receptor is an ideal approach for label-free and immune-free protein detection.