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Published on: March 26, 2015
Expression of an Activated Form of Integrin β2 Chain CD18 in Cardiac Surgical Operations
Manuela Kudlová1, Martina Koláčková1, Pavel Kuneš2,1
1Charles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Clinical Immunology and Allergology, Hradec Králové, Czech Republic.
Background:
Myeloid cells are extensively activated in patients undergoing cardiosurgical operations. It is supposed that this activation is more profound in patients operated with cardiopulmonary bypass (CPB) ("on-pump") in comparison with patients operated without CPB ("off-pump").
Aims:
To evaluate changes in the expression of a novel activation marker expressed on myeloid cells recognized by MEM-148 antibody.
Patients And Methods:
The expression of MEM-148 positive myeloid cells was evaluated by flow cytometry in 40 patients who underwent coronary artery bypass surgery (CABG) randomly assigned to "on-pump" or "off-pump" technique.
Results:
The relative and absolute number of MEM-148 positive myeloid cells is significantly diminished during "on-pump" surgery. A significant increase in their number in postoperative period in both "on-pump" and "off-pump" patients was found. There were no significant differencies between "on-pump" and "off-pump" patients.
Conclusions:
The very trauma of surgery seems to be more relevant in starting on activation of myeloid cells them CPB itself.
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