Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prenatal β-catenin/Brn2/Tbr2 transcriptional cascade regulates adult social and stereotypic behaviors.

Molecular psychiatry·2016
Same author

PI3K/AKT signaling determines a dynamic switch between distinct KSRP functions favoring skeletal myogenesis.

Cell death and differentiation·2011
Same author

Akt2-mediated phosphorylation of Pitx2 controls Ccnd1 mRNA decay during muscle cell differentiation.

Cell death and differentiation·2009
Same author

Pitx genes during cardiovascular development.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis.

Genes & development·2001
Same author

The effect of a BRN 3.1 deletion on the temperature response to 2G.

Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2001
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expression.

S E Leff, R M Evans, M G Rosenfeld

    Cell
    |February 13, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Cell-specific RNA processing of the calcitonin/CGRP gene yields distinct messenger RNAs (mRNAs) in neurons and thyroid cells. This study suggests a neural factor dictates alternative splicing and polyadenylation for CGRP mRNA production.

    More Related Videos

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    Related Experiment Videos

    Last Updated: May 10, 2026

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
    10:50

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

    Published on: April 24, 2021

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Genetics

    Background:

    • The calcitonin/CGRP gene undergoes cell-specific alternative RNA processing.
    • This results in distinct messenger RNAs (mRNAs): CGRP mRNA in neurons and calcitonin mRNA in thyroid C cells.
    • Alternative polyadenylation and exon splicing are key mechanisms driving transcript diversity.

    Purpose of the Study:

    • To investigate the regulatory mechanisms underlying cell-specific alternative RNA processing of the calcitonin/CGRP gene.
    • To identify factors or machinery responsible for differential mRNA production in neurons versus thyroid cells.
    • To explore the role of splice commitment regulatory factors in CGRP mRNA generation.

    Main Methods:

    • Expression of wild-type and mutated calcitonin/CGRP genes in heterologous cell systems.
    • Analysis of resulting mRNA populations to determine patterns of alternative polyadenylation and splicing.
    • Comparative studies between neuronal and thyroid cell expression patterns.

    Main Results:

    • Heterologous expression predominantly produced either calcitonin or CGRP mRNA, depending on the cell type.
    • Evidence suggests neurons possess specific machinery or factors influencing alternative splicing pathways.
    • A correlation was observed between specific splicing pathways and alternative poly(A) site selection.

    Conclusions:

    • Neurons likely express a regulatory factor that directs alternative splicing and polyadenylation for CGRP mRNA production.
    • It is hypothesized that a splice commitment regulatory factor modulates pre-mRNA structure.
    • This modulation may reveal cryptic splice sites essential for generating brain-specific CGRP mRNA.