Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-Omics Profiling Uncovers Predictive Factors for Tumor-Infiltrating Lymphocyte Therapy in Ovarian Cancer.

Journal of clinical laboratory analysis·2026
Same author

Cadonilimab plus chemotherapy with or without bevacizumab as first-line treatment for advanced cervical cancer: subgroup analyses from the COMPASSION-16 phase 3 trial.

Nature communications·2026
Same author

CDO1 and CELF4 methylation assay as the dominant predictor of endometrial cancer: A cohort analysis across pre- and post-menopausal cohorts.

Gynecologic oncology·2026
Same author

A stabilized MERS-CoV spike ferritin nanoparticle vaccine elicits robust and protective neutralizing antibody responses.

Nature communications·2026
Same author

Structure and immunogenicity of an engineered soluble prefusion-stabilized EBV gB antigen.

Nature communications·2026
Same author

Case Report: Rare Müllerian malformation assisted by digital 3D CT reconstruction technology for diagnosis and treatment.

Frontiers in medicine·2025

Related Experiment Video

Updated: Feb 24, 2026

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
07:42

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

7.9K

A selective insecticidal protein from Pseudomonas mosselii for corn rootworm control.

Jun-Zhi Wei1, Jessica O'Rear1, Ute Schellenberger1

  • 1DuPont Pioneer, Hayward, CA, USA.

Plant Biotechnology Journal
|August 11, 2017
PubMed
Summary
This summary is machine-generated.

A new insecticidal protein, PIP-47Aa, from Pseudomonas mosselii effectively controls western corn rootworm (WCR) and other pests. It offers a novel solution for resistant WCR populations, unlike current Bacillus thuringiensis (Bt) toxins.

Keywords:
DiabroticaPseudomonasinsecticidal proteinmode of actionwestern corn rootworm

More Related Videos

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
09:42

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

10.6K
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids
08:28

Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids

Published on: July 21, 2023

2.6K

Related Experiment Videos

Last Updated: Feb 24, 2026

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
07:42

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

7.9K
Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
09:42

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

10.6K
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids
08:28

Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids

Published on: July 21, 2023

2.6K

Area of Science:

  • Agricultural Entomology
  • Molecular Biology
  • Biochemistry

Background:

  • Western corn rootworm (WCR) is a major agricultural pest, with resistance evolving to current Bacillus thuringiensis (Bt) crop defenses.
  • Novel insecticidal proteins are needed to manage WCR populations resistant to existing Bt toxins.

Purpose of the Study:

  • To discover and characterize a novel insecticidal protein effective against WCR.
  • To evaluate the efficacy of PIP-47Aa against WCR and other beetle pests, including resistant strains.

Main Methods:

  • Discovery of PIP-47Aa from Pseudomonas mosselii.
  • Expression of recombinant PIP-47Aa and testing its toxicity against various insect species.
  • Development of transgenic corn plants expressing PIP-47Aa and assessment of root protection against WCR.

Main Results:

  • PIP-47Aa demonstrated potent insecticidal activity against WCR, other Diabrotica species, and Phyllotreta cruciferae.
  • PIP-47Aa was not toxic to beneficial insects like Coleomegilla maculata or various Lepidoptera and Hemiptera.
  • Transgenic corn expressing PIP-47Aa provided significant protection against WCR root damage.
  • PIP-47Aa effectively killed mCry3A-resistant WCR and lacks shared binding sites with mCry3A or AfIP-1A/1B.

Conclusions:

  • PIP-47Aa represents a novel class of insecticidal proteins with potential for WCR management.
  • This protein offers a new tool for controlling corn rootworm pests, including those resistant to current Bt toxins.