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Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice
Published on: March 15, 2019
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Hyperoxia does not directly affect vascular tone in isolated arteries from mice
B Smit1, Y M Smulders2, M C de Waard1
1Department of Intensive Care, VU University Medical Center, Amsterdam, the Netherlands.
Plos One
|August 11, 2017
Summary
High oxygen levels (hyperoxia) did not alter blood vessel tone in isolated mouse arteries. This study found that mouse arteries are not sensitive to hyperoxia, suggesting this model is unsuitable for studying hyperoxia-induced vasoconstriction.
Area of Science:
- Physiology
- Vascular Biology
- Medical Research
Background:
- Hospitalized patients often receive oxygen therapy, potentially causing hyperoxia (supraphysiological oxygen tension).
- Hyperoxia is linked to hemodynamic changes like increased systemic vascular resistance, suggesting vasoconstriction, but direct evidence is inconsistent.
- Mouse models are crucial for disease research, yet hyperoxia's direct vascular effects remain unstudied in mice.
Purpose of the Study:
- To develop and validate an ex-vivo pressure-myograph model using mouse arteries to investigate hyperoxia-induced vasoconstriction.
- To assess the direct impact of varying hyperoxic conditions on vascular tone in isolated mouse arteries.
- To determine if hyperoxia affects vascular responses to vasodilators and vasoconstrictors.
Main Methods:
- Isolated conduit (femoral artery) and resistance (gracilis arteriole) arteries from C57BL/6 mice were used.
- A no-flow pressure myograph system exposed vessels to normoxia (80 mmHg) and hyperoxia (215, 375, 665 mmHg).
- Vessel responses to endothelium-dependent/independent vasodilators (acetylcholine, arachidonic acid, s-nitroprusside) and vasoconstrictors (norepinephrine, prostaglandin F2α) were evaluated.
Main Results:
- Exposed mouse arteries and arterioles showed no change in vascular tone under normoxic or hyperoxic conditions.
- Hyperoxia did not alter the maximal responses or EC50 values to any tested vasodilators or vasoconstrictors.
- The developed pressure-myograph model did not detect intrinsic sensitivity of mouse vessels to hyperoxia.
Conclusions:
- Arteries and arterioles from healthy mice do not exhibit intrinsic sensitivity to hyperoxic conditions.
- The ex-vivo pressure-myograph model, as applied, is not suitable for researching the mechanisms of hyperoxic vasoconstriction in mice.
- Further investigation is needed to understand hyperoxia's vascular effects, potentially using different models or conditions.
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