Quantitative response relationships between net nitrogen transformation rates and nitrogen functional genes during
Honglei Wang1, Na Deng2, Duoyang Wu2
1State Key Laboratory of Soil Erosion and Dry land Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A & F University, Yangling, 712100, Shaanxi, China. wanghonglei@nwsuaf.edu.cn.
Abstract:
A comprehensive understanding of how microbial associated with nitrogen (N) cycling respond to artificial vegetation restoration is still lacking, particularly in arid to semi-arid degraded ecosystems. We compared soil net N mineralization rates and the abundance of bacteria, archaea, and eleven N microbial genes on the northern Loess Plateau of China during the process of artificial vegetation restoration. The quantitative relationships between net N mineralization rates and N microbial genes were determined. We observed a significant difference of net transformation rates of NH4+-N (Ra), NO3--N (Rd), and total mineralization (Rm), which rapidly decreased in 10-year soils and steadily increased in the 10-30-year soils. Different N functional microbial groups responded to artificial vegetation restoration distinctly and differentially, especially for denitrifying bacteria. Stepwise regression analysis suggested that Ra was collectively controlled by AOA-amoA and Archaea; Rd was jointly governed by narG, napA, nxrA, and bacreria; and Rm was jointly controlled by napA, narG, nirK, nirS, norB, nosZ, and nxrA.
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