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Vancomycin-resistant gene identification from live bacteria on an integrated microfluidic system by using low
Wen-Hsin Chang1, Ju-Ching Yu1, Sung-Yi Yang2
1Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Biomicrofluidics
|August 12, 2017
Summary
A new microfluidic system rapidly detects the vancomycin-resistant gene (vanA) in live bacteria from clinical samples within one hour. This advancement offers a sensitive and accurate method for monitoring antibiotic resistance, crucial for infection control.
Area of Science:
- Microbiology
- Molecular Biology
- Biotechnology
Background:
- Vancomycin-resistant Enterococcus (VRE) poses a significant threat due to rising resistance rates and gene transmission.
- Current diagnostic methods for VRE are time-consuming, lack genetic information, or have high false-positive rates.
- Timely diagnosis and control of VRE are essential for preventing patient infections.
Purpose of the Study:
- To develop an integrated microfluidic system for automated detection of the vanA gene in live bacteria.
- To establish a rapid, sensitive, and accurate diagnostic tool for vancomycin resistance in clinical settings.
Main Methods:
- An integrated microfluidic system was developed for automated detection.
- The system utilizes ethidium monoazide, nucleic acid probes, chemical lysis, and loop-mediated isothermal amplification (LAMP).
- Detection was performed on live Enterococcus in joint fluid samples.
Main Results:
- The system successfully detected the vanA gene from live Enterococcus in joint fluid samples.
- A low detection limit of 10 colony-forming units/reaction was achieved.
- The entire process was completed within 1 hour.
Conclusions:
- This study presents the first integrated microfluidic system using LAMP to detect the vanA gene from live bacteria.
- The developed system demonstrates high sensitivity and accuracy.
- The system holds potential for monitoring antibiotic resistance genes in clinical samples.
