Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Steady, Laminar Flow Between Parallel Plates

Steady, Laminar Flow Between Parallel Plates

Understanding steady, laminar flow between parallel plates is essential for analyzing and designing flow in narrow rectangular channels, commonly found in various water conveyance and drainage systems. The Navier-Stokes equations govern fluid motion and are generally challenging to solve due to their nonlinearity. However, simplifications are possible in certain cases, like the steady laminar flow between parallel plates. For this scenario, we assume steady, incompressible, laminar flow.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Magnetophoresis in Centrifugal Microfluidics at Continuous Rotation for Nucleic Acid Extraction.

Micromachines·2022

Same author

Holographic PIV/PTV for nano flow rates-A study in the 70 to 200 nL/min range.

Biomedizinische Technik. Biomedical engineering·2022

Same author

Automation of peptide desalting for proteomic liquid chromatography - tandem mass spectrometry by centrifugal microfluidics.

Lab on a chip·2021

Same author

Tryptic digestion of human serum for proteomic mass spectrometry automated by centrifugal microfluidics.

Lab on a chip·2020

Same author

Library preparation for next generation sequencing: A review of automation strategies.

Biotechnology advances·2020

Same author

Review on pneumatic operations in centrifugal microfluidics.

Lab on a chip·2019

Search research articles

Related Experiment Video

Updated: Feb 24, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Network simulation-based optimization of centrifugo-pneumatic blood plasma separation.

S Zehnle¹, M Rombach¹, R Zengerle

¹Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany.

Biomicrofluidics

|August 12, 2017

Summary

This study presents a rapid, automated method for separating plasma from whole blood using a microfluidic disk. The technique achieves high plasma purity, enabling efficient blood sample processing.

More Related Videos

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Published on: February 1, 2022

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Related Experiment Videos

Last Updated: Feb 24, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Published on: February 1, 2022

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Area of Science:

Biomedical Engineering
Microfluidics
Clinical Diagnostics

Background:

Accurate plasma separation is crucial for various diagnostic assays.
Existing methods can be time-consuming or require complex instrumentation.
Microfluidic devices offer potential for rapid and automated sample processing.

Purpose of the Study:

To develop and demonstrate an automated, robust, and rapid plasma separation technique using centrifugal microfluidics.
To achieve high plasma purity suitable for downstream analysis.
To optimize the microfluidic disk design and operational protocol for efficiency and insusceptibility to red blood cell resuspension.

Main Methods:

Utilized a centrifugal microfluidic polymer disk for blood separation.
Employed a two-step rotational frequency protocol to concentrate red blood cells (RBCs) and plasma.
Optimized disk design and pneumatic actuation for plasma transfer and RBC suppression.
Assessed plasma purity by measuring residual hemoglobin after RBC lysis.

Main Results:

Achieved automated separation of 14 μl plasma from 40 μl whole blood within 43 seconds.
Demonstrated high plasma purity of 99.81% ± 0.11% across a hematocrit range of 20%-60%.
The pneumatic actuation eliminated the need for surface treatments or external means, facilitating low-cost fabrication.

Conclusions:

The developed centrifugal microfluidic system provides a fast, robust, and highly pure plasma separation method.
The pneumatic actuation and optimized protocol enable efficient processing without significant red blood cell contamination.
This technology holds promise for low-cost, mass-produced diagnostic devices, particularly for point-of-care applications.