Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.7K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.7K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.2K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

2.4K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.4K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.7K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.7K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.5K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.5K
Chromatography: Introduction01:10

Chromatography: Introduction

7.6K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homozygous familial hypercholesterolemia (HoFH) in Canada.

Atherosclerosis·2026
Same author

Aortic Stenosis in Homozygous Familial Hypercholesterolemia: The Canadian HoFH Registry.

JACC. Advances·2026
Same author

What is effective communication in breastfeeding care? Perspectives from Latina women.

PloS one·2025
Same author

Ionic Twin Nanostructural Comparison: Propylammonium Butanoate vs. Butylammonium Propanoate and Their Interactions with Water.

Materials (Basel, Switzerland)·2024
Same author

Homozygous Familial Hypercholesterolemia in Canada: An Observational Study.

JACC. Advances·2024
Same author

Sex differences in the presentation, treatment and outcomes of patients with homozygous familial hypercholesterolemia.

Journal of clinical lipidology·2024
Same journal

AI-Driven Design Platforms of Next-Generation Antibody Therapeutics.

Topics in current chemistry (Cham)·2026
Same journal

Progress and Challenges in Chemical Looping Hydrogen Production Technology and Oxygen Carrier Development: A Review.

Topics in current chemistry (Cham)·2026
Same journal

Multicomponent Reactions for the Synthesis of Oxazepines.

Topics in current chemistry (Cham)·2026
Same journal

Advances in Ciprofloxacin Derivatives: Emerging Strategies to Combat Antimicrobial Resistance.

Topics in current chemistry (Cham)·2026
Same journal

C-H Nitrooxylation: A Shortcut to Nitrate Esters.

Topics in current chemistry (Cham)·2026
Same journal

Harnessing Organocatalysis for Enantioselective Chromane Synthesis.

Topics in current chemistry (Cham)·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.7K

Ionic Liquid-Liquid Chromatography: A New General Purpose Separation Methodology.

Leslie Brown1, Martyn J Earle2, Manuela A Gîlea3

  • 1AECS-QuikPrep Ltd, 55 Gower Street, London, WC1 6HQ, UK.

Topics in Current Chemistry (Cham)
|August 12, 2017
PubMed
Summary
This summary is machine-generated.

Ionic liquids create biphasic systems for advanced liquid-liquid separations and countercurrent chromatography. Tailored ionic liquids enable novel separations of diverse organic, inorganic, and bio-based materials.

Keywords:
Countercurrent chromatographyIonic liquidsIonic liquid–liquid chromatographySeparationsSolvent engineering

More Related Videos

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.8K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.6K

Related Experiment Videos

Last Updated: Feb 24, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.7K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.8K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.6K

Area of Science:

  • Green chemistry
  • Separation science
  • Analytical chemistry

Background:

  • Ionic liquids (ILs) form biphasic solvent systems with organic solvents and water.
  • These systems are applicable to liquid-liquid separations and countercurrent chromatography.
  • A wide array of synthesizable ILs offers tailored properties for material separation.

Purpose of the Study:

  • To explore the application of ionic liquid-based biphasic systems in advanced separation techniques.
  • To develop and utilize a customized countercurrent chromatograph for enhanced separation capabilities.
  • To demonstrate the versatility of ionic liquids in separating diverse organic, inorganic, and bio-based materials.

Main Methods:

  • Formation of biphasic solvent systems using various ionic liquids, organic solvents, and water.
  • Design and construction of a specialized countercurrent chromatograph to accommodate viscous ionic liquid systems.
  • Application of the developed system for the separation of transition metal salts, arenes, alkenes, alkanes, bio-oils, and sugars.

Main Results:

  • Successful implementation of ionic liquid-based biphasic systems in liquid-liquid separations.
  • Demonstrated effectiveness of the customized countercurrent chromatograph in handling viscous ILs.
  • Achieved efficient separation of a wide range of target compounds, showcasing the system's versatility.

Conclusions:

  • Ionic liquids offer a novel and versatile platform for designing advanced separation processes.
  • Customized countercurrent chromatography is a viable technique for exploiting the properties of IL-based solvent systems.
  • This approach provides a new philosophy for the separation of complex organic, inorganic, and bio-based materials.