Related Experiment Video

Updated: Feb 24, 2026

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
09:10

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Published on: April 21, 2023

1.5K

SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway

Ning Xie1,2, Min Chen1, Rilei Dai1

  • 1Capital Institute of Pediatrics, Beijing 100020, China.

Nature Communications
|August 12, 2017
PubMed

Insights

Serine/arginine-rich splicing factor 1 (SRSF1) drives vascular smooth muscle cell proliferation and cardiovascular disease. Targeting SRSF1 offers a new therapeutic strategy for vascular hyperplastic disorders.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Cellular Biology

Background:

  • Vascular smooth muscle cell (VSMC) proliferation is central to cardiovascular hyperplastic disorders.
  • The precise molecular drivers of VSMC proliferation remain incompletely understood.

Purpose of the Study:

  • To investigate the role of serine/arginine-rich splicing factor 1 (SRSF1) in VSMC proliferation and neointima formation.
  • To elucidate the molecular mechanisms by which SRSF1 influences VSMC behavior.

Main Methods:

  • Assessed SRSF1 expression in response to vascular injury and proliferative stimuli.
  • Utilized SRSF1 knockout mouse models and in vitro VSMC cultures.
  • Examined the impact of SRSF1 modulation on cell proliferation, migration, and gene expression.

Main Results:

  • SRSF1 expression is upregulated by vascular injury and mitogens.
  • SMCs lacking SRSF1 exhibit reduced neointima formation post-injury.
  • SRSF1 overexpression promotes, while knockdown inhibits, VSMC proliferation and migration.
  • SRSF1 promotes a truncated p53 isoform (Δ133p53), activating KLF5 via the Δ133p53-EGR1 complex, accelerating cell cycle progression.

Conclusions:

  • SRSF1 is a key regulator of VSMC proliferation and neointima formation.
  • The SRSF1/Δ133p53/KLF5 pathway is critical for accelerated cell-cycle progression in VSMCs.
  • SRSF1 represents a potential therapeutic target for vascular hyperplastic diseases.

More Related Videos

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.7K
Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

8.8K

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.7K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.8K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K