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Published on: April 10, 2018
Dengue virus isolation by antibody-dependent enhancement of infectivity in macrophages
Abstract:
Acute-phase serum samples collected during an outbreak of dengue fever and dengue haemorrhagic fever in Penang, Malaysia, were tested by a method involving antibody-dependent enhancement of infectivity in the mouse macrophage-like cell line, P388D1. 58 of 71 (81.7%) serologically positive cases yielded virus.
Insights
Researchers identified dengue virus in 81.7% of patients during a Malaysian outbreak. This study utilized antibody-dependent enhancement of infectivity in cell cultures to detect the virus in acute-phase serum samples.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Dengue fever and dengue hemorrhagic fever pose significant public health challenges.
- Accurate and timely diagnosis is crucial for managing dengue outbreaks.
Purpose of the Study:
- To evaluate a diagnostic method for detecting dengue virus in patient samples.
- To determine the efficiency of virus isolation from acute-phase serum during an outbreak.
Main Methods:
- Acute-phase serum samples from a dengue outbreak in Penang, Malaysia were analyzed.
- A cell culture method using antibody-dependent enhancement of infectivity was employed.
- The mouse macrophage-like cell line P388D1 was used for virus isolation.
Main Results:
- Virus was successfully isolated from 58 out of 71 (81.7%) serologically positive cases.
- The method demonstrated high efficiency in detecting dengue virus in acute samples.
Conclusions:
- Antibody-dependent enhancement of infectivity in P388D1 cells is an effective method for dengue virus detection.
- This technique can be valuable for diagnosing dengue during outbreaks.
