Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Muscle Regeneration Failure May Lead to Clinical Features of Myopathy in Anti-IgLON5 Disease.

Neurology(R) neuroimmunology & neuroinflammation·2026
Same author

The functional organization of retinal input to the mouse superior colliculus.

bioRxiv : the preprint server for biology·2026
Same author

Distribution and functional significance of rodent cerebellar glycogen.

iScience·2026
Same author

Developmental regulation of GABA<sub>B</sub> receptors and downstream molecules in the mouse brain.

Histology and histopathology·2025
Same author

A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics.

Nature neuroscience·2025
Same author

Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons.

PLoS biology·2024

Related Experiment Video

Updated: Feb 24, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

14.0K

The genetic encoded toolbox for electron microscopy and connectomics.

Ryuichi Shigemoto1, Maximilian Joesch1

  • 1IST Austria, Klosterneuburg, Austria.

Wiley Interdisciplinary Reviews. Developmental Biology
|August 12, 2017
PubMed
Summary

Genetically encoded probes enhance electron microscopy for ultrastructural mapping. These tools accelerate the analysis of neural circuits and synaptic connections, significantly advancing connectomics research.

More Related Videos

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.5K
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
07:33

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

11.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

14.0K
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.5K
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
07:33

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

11.5K

Area of Science:

  • Bioengineering
  • Molecular Biology
  • Neuroscience

Background:

  • Electron microscopy (EM) is crucial for ultrastructural analysis.
  • Identifying specific cell populations and their connections in EM is challenging.
  • Current methods lack efficiency in mapping complex neural circuits.

Purpose of the Study:

  • To introduce genetically encoded probes for enhanced EM.
  • To detail their application in ultrastructural mapping of neural circuits.
  • To highlight their impact on connectomics and structure-function studies.

Main Methods:

  • Development of genetically encoded probes targeting specific cellular compartments.
  • Utilizing probes to create electron-dense markers via chemical reactions.
  • Application of these probes in electron microscopy for enhanced contrast and identification.

Main Results:

  • Probes enable genetic identification of circuit components.
  • Facilitate targeted imaging of specific regions of interest.
  • Allow for automated analysis of tagged neural circuits, reducing analysis time by over two orders of magnitude.

Conclusions:

  • Genetically encoded tags for EM simplify circuit motif analysis.
  • These probes are poised to become essential tools for structure-function studies of synaptic connections.
  • Significant advancements in connectomics are expected.