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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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MicroRNA expression in bone marrow-derived human multipotent Stromal cells.

Ian H Bellayr1, Abhinav Kumar1, Raj K Puri2

  • 1Tumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics and Evaluation Research, US Food and Drug Administration, Silver Spring, MD, USA.

BMC Genomics
|August 13, 2017
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) like miR-638 and miR-572 show altered expression in multipotent stromal cells (MSCs) across different passages. These specific miRNAs can help distinguish MSCs at various stages of cell culture.

Keywords:
MicroRNA expressionMicroarrayMultipotent Stromal cells

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Area of Science:

  • Stem Cell Biology
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Multipotent stromal cells (MSCs) are crucial in regenerative medicine due to their differentiation and immunoregulatory properties.
  • MicroRNAs (miRNAs) regulate gene expression and influence MSC functions like proliferation, differentiation, migration, and cell death.
  • The specific impact of miRNAs on MSC functions and donor variability remains incompletely understood.

Purpose of the Study:

  • To investigate the impact of cell culture passages on miRNA expression in MSCs.
  • To identify specific miRNAs whose expression levels change significantly with MSC expansion.
  • To assess the potential of these miRNAs as biomarkers for MSC quality attributes.

Main Methods:

  • Eight MSC lines were cultured and expanded to passages 3, 5, and 7.
  • Microarray technology was employed to evaluate miRNA expression profiles across passages.
  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to validate findings for specific miRNAs in independent MSC sets.

Main Results:

  • Out of 939 miRNAs examined, 71 were consistently expressed across all passages.
  • Significant differences in miRNA expression were observed between passages 3 and 7 (11 miRNAs) and passages 3 and 5 (7 miRNAs).
  • RT-qPCR confirmed upregulation of miR-638 and miR-572 in later passages compared to earlier ones in two independent MSC sets.

Conclusions:

  • The expression patterns of miR-638 and miR-572 can differentiate MSCs from distinct cell culture passages.
  • These findings suggest miR-638 and miR-572 could serve as critical quality attributes for MSCs.
  • Further research is warranted to determine if changes in these miRNAs affect essential MSC functions.