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Knockdown of eukaryotic translation initiation factor 3 subunit D (eIF3D) inhibits proliferation of acute myeloid

Guo-Zhen Liu1, Ji-Zhu Liu1, Xiao-Qing Li2

  • 1Department of Hematology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, People's Republic of China.

Molecular and Cellular Biochemistry
|August 13, 2017
PubMed

Insights

Eukaryotic translation initiation factor 3 subunit D (eIF3D) drives acute myeloid leukemia (AML) cell proliferation. Silencing eIF3D inhibits AML cell growth, suggesting it as a potential therapeutic target for leukemia.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Hematology

Background:

  • Eukaryotic translation initiation factors (eIFs) are linked to cancer.
  • Eukaryotic translation initiation factor 3 subunit D (eIF3D) impacts human cancer cell growth.
  • The role of eIF3D in acute myeloid leukemia (AML) is not well understood.

Purpose of the Study:

  • To investigate the expression of eIF3D in AML cell lines.
  • To determine the functional role of eIF3D in AML cell proliferation.
  • To evaluate eIF3D as a potential therapeutic target in AML.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot analysis to confirm eIF3D knockdown.
  • Lentivirus-mediated RNA interference to silence eIF3D expression in U937 cells.
  • Flow cytometry to analyze cell cycle progression and apoptosis.

Main Results:

  • eIF3D was expressed in all tested leukemia cell lines.
  • eIF3D knockdown significantly inhibited U937 cell proliferation.
  • Silencing eIF3D induced G2/M phase cell cycle arrest and apoptosis.

Conclusions:

  • eIF3D is crucial for the proliferation of AML cells.
  • Targeting eIF3D through silencing presents a potential therapeutic strategy for leukemia.
Keywords:
Acute myeloid leukemiaApoptosisCell proliferationGrowth arrestRNA interferenceTranslational controleIF3D

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