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Updated: Feb 24, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Functional Screening of Core Promoter Activity.

Dan Y Even¹, Adi Kedmi¹, Diana Ideses¹

¹The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Life Sciences Building (212) Room 102, Ramat-Gan, Israel.

Methods in Molecular Biology (Clifton, N.J.)

|August 13, 2017

Summary

This study presents a method to analyze engineered core promoter functions using the EGFP reporter gene. This approach allows for the screening of potent core promoters for gene expression manipulation.

Keywords:

Core promoter elements Engineered promoters FACS analysis Live cell imaging Reporter gene analysis

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Last Updated: Feb 24, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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Published on: March 3, 2015

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

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Published on: June 1, 2014

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

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Published on: May 27, 2016

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Core promoters are crucial DNA sequences regulating gene transcription initiation.
Engineered core promoters offer potential for precise control of gene expression levels.

Purpose of the Study:

To describe a methodology for the qualitative and quantitative analysis of engineered core promoter functions.
To enable the screening of potent core promoters for gene expression manipulation.

Main Methods:

Utilizing an EGFP reporter gene driven by distinct engineered core promoters.
Transfecting expression plasmids into mammalian cell lines for fluorescence monitoring via live cell imaging and flow cytometry.
Performing real-time quantitative PCR to confirm transcriptional activity independent of DNA uptake differences.

Main Results:

Demonstrated a robust method for analyzing engineered core promoter activity.
Successfully screened potent engineered core promoters for extended functional analysis.
Validated the accuracy of the methodology in assessing promoter-specific transcriptional output.

Conclusions:

The described methodology provides a reliable approach for functional screening of engineered core promoter activity.
This technique facilitates the rational design and optimization of core promoters for gene expression control.
The study highlights the potential of engineered promoters in biotechnology and synthetic biology applications.