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MicroRNA-492 overexpression involves in cell proliferation, migration, and radiotherapy response of cervical squamous

Mei Liu1, Jusheng An2, Manni Huang2

  • 1Laboratory of Cell and Molecular Biology & State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.

Molecular Carcinogenesis
|August 13, 2017
PubMed

Insights

MicroRNA 492 (miR-492) is upregulated in cervical cancer, correlating with treatment sensitivity and lymph node metastasis. It enhances cell proliferation and radiation sensitivity, potentially serving as a biomarker for cervical cancer prognosis and treatment response.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional regulators.
  • Cervical squamous cell carcinoma (CSCC) presents treatment challenges.
  • Understanding miRNA roles is crucial for targeted therapies.

Purpose of the Study:

  • To investigate the role of miR-492 in CSCC.
  • To assess miR-492 expression in relation to treatment response and metastasis.
  • To explore the functional impact of miR-492 in cervical cancer cells.

Main Methods:

  • MicroRNA profiling of 104 advanced CSCC patient biopsies.
  • Analysis of miR-492 expression in relation to chemoradiotherapy sensitivity and lymph node metastasis (LNM).
  • In vitro studies involving ectopic miR-492 overexpression in SiHa cells; target validation (TIMP2).

Main Results:

  • miR-492 was significantly higher in chemoradiotherapy-sensitive tumors and associated with pelvic LNM.
  • Overexpression of miR-492 promoted cell proliferation, migration, and enhanced radiosensitivity.
  • miR-492 directly targets TIMP2, regulating the miR-492/TIMP2/MMP10 axis in pelvic LNM.
  • miR-492 showed correlation with prognosis in negative LNM and predictive value for treatment response in positive LNM.

Conclusions:

  • miR-492 is upregulated in CSCC and linked to treatment response and metastasis.
  • miR-492 influences cervical cancer cell behavior and radiosensitivity.
  • miR-492 serves as a potential biomarker for predicting treatment outcomes and prognosis in CSCC.
Keywords:
TIMP2cervical cancermetastasismiR-492pelvic lymph noderadiosensitivity

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