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Updated: Feb 24, 2026
X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease
Published on: July 18, 2019
Phase-contrast hard X-ray microscopy using synchrotron radiation for the properties of skeletal muscle in mouse hind
Sanghun Jang1, Jaehong Lim2, Onseok Lee3
1Department of Physical Therapy, College of Nursing and Health Science, Gimcheon University, 214, Daehak-ro, Gimcheon City, Gyeongbuk 39528, South Korea.
Abstract:
Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies. We believe that this method will enhance existing morphometric methods of analysis, leading to accurate reconstruction of other thick specimens that would otherwise require thin sectioning and reconstruction through deconvolution algorithms.
