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Updated: Feb 24, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF
Laura Rindi1, Greta Alì2, Barbara Fabiani1
1Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, I-56127, Pisa, Italy.
Abstract:
GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.
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