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Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

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Published on: October 12, 2012

Laboratory Testing for Activated Protein C Resistance (APCR).

Soma Mohammed¹, Emmanuel J Favaloro²

¹Haematology Department, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW, 2145, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|August 15, 2017

Summary

Activated protein C resistance (APCR) is a disorder increasing thrombosis risk due to impaired anticoagulant response. Testing involves automated assays to detect this condition, crucial for managing venous thromboembolism.

Keywords:

Activated Protein C resistance (APCR)Protein C (PC)Russell Viper Venom Time (RVVT)

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Area of Science:

Hematology
Molecular Biology
Clinical Pathology

Background:

Activated protein C resistance (APCR) is a hemostatic disorder linked to an impaired anticoagulant response to activated protein C (APC).
This condition elevates the risk of venous thrombosis, such as deep vein thrombosis and pulmonary embolism.
APCR can be hereditary, often due to Factor V Leiden mutation, or acquired, impacting thrombin generation and promoting a prothrombotic state.

Purpose of the Study:

To describe an automated laboratory procedure for detecting Activated protein C resistance (APCR).
To detail the use of a commercial Russell Viper Venom-based clotting assay for APCR assessment.
To outline the application of CS-5100 and STA-R analyzers in APCR testing.

Main Methods:

Utilized a commercial Russell Viper Venom-based clotting assay for APCR detection.
Employed automated analyzers, specifically CS-5100 and STA-R, for the assay procedure.
Focused on assessing the anticoagulant response to activated protein C (APC) in laboratory testing.

Main Results:

The study describes a validated automated method for APCR testing.
The methodology allows for efficient and reliable assessment of impaired APC function.
Results indicate the utility of the described assay in identifying patients at risk for thrombotic events.

Conclusions:

Automated assays provide a reliable method for diagnosing Activated protein C resistance (APCR).
Accurate APCR detection is vital for managing patients at risk of venous thromboembolism.
The described procedure using Russell Viper Venom-based assays on specific analyzers offers a practical diagnostic approach.