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Related Experiment Video

Updated: Feb 24, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

19.5K

One-Stage Factor VIII Assays.

Elizabeth Duncan1, Susan Rodgers2

  • 1Division of Haematology, SA Pathology, Frome Rd, Adelaide, SA, 5000, Australia. elizabeth.duncan@sa.gov.au.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2017
PubMed
Summary

This chapter details a one-stage assay for measuring Factor VIII coagulant activity (FVIII:C) and other intrinsic pathway factors. The method uses Activated Partial Thromboplastin Time (APTT) correction to diagnose deficiencies and monitor therapy.

Keywords:
CoagulationFactor IXFactor VIIIFactor XIHemophilia

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Last Updated: Feb 24, 2026

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Area of Science:

  • Hematology
  • Clinical Chemistry
  • Laboratory Medicine

Background:

  • One-stage coagulation factor assays are standard for measuring factor levels.
  • These assays are crucial for diagnosing deficiencies and guiding replacement therapy.
  • Understanding assay variations and interpretation is vital for laboratory professionals.

Purpose of the Study:

  • To describe a one-stage assay method for Factor VIII coagulant activity (FVIII:C).
  • To provide guidance for adapting the method to measure Factor IX (FIX), Factor XI (FXI), and Factor XII (FXII).
  • To discuss the impact of method variations, limitations, and result interpretation.

Main Methods:

  • Utilizes the one-stage assay methodology.
  • Measures the ability of a sample to correct the prolonged Activated Partial Thromboplastin Time (APTT) of factor-deficient plasma.
  • Compares sample correction to a known calibrator.

Main Results:

  • The described method allows for the measurement of FVIII:C.
  • The methodology can be extended to assess FIX, FXI, and FXII activity.
  • The chapter discusses factors influencing assay performance and interpretation.

Conclusions:

  • The one-stage assay is a versatile method for assessing intrinsic pathway coagulation factors.
  • Accurate measurement and interpretation are essential for clinical decision-making.
  • This chapter provides a comprehensive guide for laboratory practitioners using these assays.