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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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Assessment of Factor XIII.

László Muszbek1, Éva Katona2, Adrienne Kerényi3

  • 1Division of Clinical Laboratory Science, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, Hungary. muszbek@med.unideb.hu.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2017
PubMed
Summary

Diagnosing blood coagulation factor XIII (FXIII) deficiency requires specific tests beyond standard screening. This study details key assays for FXIII activity, antigen levels, and antibody detection.

Keywords:
Factor XIIIFactor XIII activity assayFactor XIII-A antigen assayFactor XIII-A2B2 antigen assayFibrin cross-linking assayInhibitory anti-FXIII-A antibodyNeutralizing antibody

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Area of Science:

  • Hematology
  • Hemostasis and Thrombosis

Background:

  • Blood coagulation factor XIII (FXIII) is critical for hemostasis.
  • FXIII deficiency causes severe bleeding diathesis, often fatal without prophylaxis.
  • Standard hemostasis screening tests are typically normal in FXIII deficiency.

Purpose of the Study:

  • To describe essential diagnostic methods for FXIII deficiency.
  • To detail FXIII activity assays, including the ammonia release and fibrin cross-linking methods.
  • To outline techniques for FXIII antigen measurement and antibody detection.

Main Methods:

  • Ammonia release assay for FXIII activity determination.
  • Fibrin cross-linking assay for FXIII activity.
  • ELISAs for plasma FXIII (FXIII-A2B2) and FXIII-A2 antigen.
  • Platelet FXIII activity and antigen measurement.
  • Detection and quantification of neutralizing auto- and alloantibodies.

Main Results:

  • The ammonia release assay is the primary diagnostic test for FXIII deficiency.
  • The fibrin cross-linking assay offers an alternative method for FXIII activity assessment.
  • Various assays are available for comprehensive FXIII deficiency classification and characterization.

Conclusions:

  • Accurate diagnosis of FXIII deficiency relies on specialized laboratory tests.
  • A range of methods exists for assessing FXIII activity, antigen levels, and the presence of antibodies.
  • These assays are crucial for managing patients with bleeding disorders due to FXIII deficiency.