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Updated: Feb 24, 2026
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Published on: August 14, 2017
Laboratory Testing for von Willebrand Factor Antigen (VWF:Ag).
Emmanuel J Favaloro1, Soma Mohammed2, Jürgen Patzke3
1Haematology Department, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, NSW Health Pathology, Westmead, NSW, 2145, Australia. emmanuel.favaloro@health.nsw.gov.au.
Von Willebrand disease (VWD) and acquired VWD (AVWS) stem from von Willebrand factor (VWF) issues. This paper details VWF antigen (VWF:Ag) measurement protocols using immunological assays like ELISA and LIA.
Area of Science:
- Hematology
- Clinical Chemistry
- Immunology
Background:
- Von Willebrand disease (VWD) is the most common inherited bleeding disorder.
- Acquired von Willebrand syndrome (AVWS) is another form of this condition.
- Both VWD and AVWS result from defects or deficiency in von Willebrand factor (VWF).
Purpose of the Study:
- To describe protocols for assessing VWF levels through VWF antigen (VWF:Ag) measurement.
- To provide methods for quantitative assessment of VWF protein.
Main Methods:
- Utilizes immunological assays for VWF:Ag assessment.
- Details enzyme-linked immunosorbent assays (ELISA).
- Describes latex-enhanced immunoassays (LIA).
Main Results:
- The paper outlines established protocols for VWF:Ag measurement.
- ELISA and LIA are identified as common methods for VWF:Ag quantification.
Conclusions:
- Accurate VWF level assessment is crucial for diagnosing VWF-related disorders.
- Standardized protocols for VWF:Ag assays ensure reliable VWF level determination.
- Immunoassays like ELISA and LIA are key for quantifying VWF protein levels.
