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Laboratory Testing for von Willebrand Factor: Factor VIII Binding (for 2N VWD)
Soma Mohammed1, Emmanuel J Favaloro2
1Department of Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW, 2145, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2017
Summary
This study details a protocol for assessing von Willebrand factor (VWF) activity using VWF: factor VIII binding (VWF:FVIIIB) assays. This method is crucial for diagnosing type 2N VWD by measuring VWF
Area of Science:
- Hematology
- Clinical Chemistry
- Biochemistry
Background:
- Von Willebrand disease (VWD) is the most common inherited bleeding disorder, with acquired forms (AVWS) also existing.
- VWD arises from defects or deficiency in von Willebrand factor (VWF), a key plasma protein.
- Accurate diagnosis requires assessing both VWF levels and VWF activity, necessitating multiple assays due to VWF's complex functions.
Purpose of the Study:
- To describe a protocol for assessing VWF activity through VWF: factor VIII binding (VWF:FVIIIB).
- To highlight the assay's importance in identifying or excluding type 2N VWD.
- To present a specific enzyme-linked immunosorbent assay (ELISA) protocol for VWF:FVIIIB measurement.
Main Methods:
- Quantitative assessment of VWF protein adhesion to FVIII.
- Utilizing enzyme-linked immunosorbent assays (ELISA) for VWF:FVIIIB measurement.
- Detailed protocol description for VWF:FVIIIB assay implementation.
Main Results:
- The VWF:FVIIIB assay quantifies VWF's ability to bind FVIII.
- This binding activity is specifically lost in type 2N VWD.
- The described ELISA protocol provides a method for this quantitative assessment.
Conclusions:
- The VWF:FVIIIB assay is critical for diagnosing type 2N VWD.
- This protocol offers a standardized method for assessing a key VWF activity.
- ELISA-based VWF:FVIIIB assays are valuable tools in VWD diagnostics.
