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The Histone Methyltransferase Mixed Lineage Leukemia (MLL) 3 May Play a Potential Role on Clinical Dilated

Ding-Sheng Jiang1,2,3, Xin Yi4,5,6, Rui Li1

  • 1Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Molecular Medicine (Cambridge, Mass.)
|August 15, 2017
PubMed

Insights

Histone methyltransferase MLL3 is elevated in dilated cardiomyopathy (DCM), potentially driving disease progression by altering histone marks and key protein levels. This finding offers new insights into DCM pathogenesis.

Area of Science:

  • Cardiovascular Biology
  • Epigenetics
  • Molecular Medicine

Background:

  • Histone modifications are crucial in dilated cardiomyopathy (DCM) pathogenesis.
  • The specific roles of histone methyltransferases (HMTs), particularly the mixed lineage leukemia (MLL) family, in DCM remain largely unknown.

Purpose of the Study:

  • To investigate the expression patterns and functional significance of MLL family members in DCM.
  • To explore the relationship between MLL3 expression and cardiac remodeling markers in DCM.

Main Methods:

  • Analysis of MLL family gene and protein expression in human DCM and normal heart samples.
  • Induction of cardiac remodeling in a mouse model using transverse aortic constriction (TAC).
  • Quantitative real-time PCR and Western blotting to assess gene and protein expression levels.

Main Results:

  • MLL3 mRNA and protein levels were significantly increased in DCM hearts and TAC-induced mouse hearts.
  • MLL3 expression correlated with left ventricular end-systolic diameter and ejection fraction.
  • Elevated levels of di-methylated histone H3 lysine 4 (H3K4me2) and specific regulatory proteins (Smad3, GATA4, EGR1) were observed in DCM hearts.

Conclusions:

  • MLL3 plays a potential role in the pathological processes of DCM.
  • MLL3 may exert its effects by regulating H3K4me2 levels and the expression of Smad3, GATA4, and EGR1.
  • These findings highlight MLL3 as a potential therapeutic target for DCM.

Keywords:
cardiologycardiovascular diseasegene expressionpathologysignal transduction

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