Related Experiment Video

Updated: Aug 14, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
11:08

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Initial glycosylation of proteins with acetylgalactosaminylserine linkages

G J Strous

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1979
    PubMed

    More Related Videos

    Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins11:25

    Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

    Published on: October 4, 2017

    Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy08:16

    Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

    Published on: February 7, 2025

    Related Concept Videos

    Lipids as Anchors01:32

    Lipids as Anchors

    In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
    The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
    Protein Glycosylation01:25

    Protein Glycosylation

    Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
    Glycosylation occurs in...
    Oligosaccharide Assembly01:24

    Oligosaccharide Assembly

    Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
    Multiple sugar molecules that may or may...
    Protein Folding Quality Check in the RER01:29

    Protein Folding Quality Check in the RER

    ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
    Proteoglycans01:05

    Proteoglycans

    Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
    Glycosaminoglycans01:23

    Glycosaminoglycans

    Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
    GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
    Hyaluronic...