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Updated: Feb 24, 2026

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Aptamer Cell-Based Selection: Overview and Advances.

Silvia Catuogno1, Carla Lucia Esposito2

  • 1Istituto di Endocrinologia ed Oncologia Sperimentale "G. Salvatore", CNR, Naples 80100, Italy. s.catuogno@ieos.cnr.it.

Biomedicines
|August 15, 2017
PubMed
Summary

Cell-SELEX technology generates aptamers using living cells for diagnostics and therapeutics. This method effectively targets cell surface proteins, especially in cancer research, offering promising applications.

Keywords:
aptamercell targetingcell- Systematic Evolution of Ligands by Exponential enrichment

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Aptamers are single-stranded DNA/RNA molecules with high affinity for targets.
  • Systematic Evolution of Ligands by Exponential enrichment (SELEX) is a key production method.
  • Aptamers show potential as diagnostic and therapeutic tools.

Purpose of the Study:

  • To provide an overview of cell-SELEX technology.
  • To discuss recent advances in cell-SELEX.
  • To focus on cancer cell targeting applications.

Main Methods:

  • Cell-SELEX utilizes living cells as targets for aptamer selection.
  • This method ensures aptamer binding to native cell surface proteins.
  • It is effective for complex and modified target proteins.

Main Results:

  • Cell-SELEX has been successfully applied to generate aptamers against various cell types.
  • Recent advances focus on improving efficiency and specificity.
  • The technology is particularly promising for cancer cell targeting.

Conclusions:

  • Cell-SELEX is an effective strategy for aptamer generation against cellular targets.
  • It offers advantages for developing cancer diagnostics and therapeutics.
  • Further research and protocol optimization are ongoing.