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A comparison study on polysaccharides extracted from Laminaria japonica using different methods: structural
Jie Gao1, Lianzhu Lin, Baoguo Sun
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Food & Function
|August 15, 2017
Summary
Extraction methods significantly impact Laminaria japonica polysaccharides (LP) structure and properties. Acid-assisted extraction yielded LP with superior bile acid-binding capacity due to its unique structural characteristics.
Area of Science:
- Marine biotechnology
- Food science
- Biochemistry
Background:
- Laminaria japonica polysaccharides (LP) are known for potential health benefits.
- Understanding the impact of extraction methods on LP properties is crucial for optimizing their application.
- Bile acid-binding capacity is a key indicator of hypolipidemic activity.
Purpose of the Study:
- To investigate the effect of seven different extraction methods on the structural characteristics of Laminaria japonica polysaccharides (LP).
- To evaluate the bile acid-binding capacity of LP extracted using various methods.
- To establish a method for assessing the hypolipidemic potential of LP.
Main Methods:
- Extraction of LP using seven distinct methods.
- Structural characterization including molecular weight, monosaccharide composition, and AFM analysis.
- Rheological measurements to determine viscosity.
- In vitro assessment of bile acid-binding capacity.
Main Results:
- Extraction methods significantly influenced LP yield, molecular weight, monosaccharide composition, and uronic acid/fucose content.
- AFM revealed differences in chain flexibility and branching among LP samples.
- LP extracted via pressurized hot water and acid-assisted methods showed lower viscosity.
- Acid-assisted extracted LP exhibited the highest bile acid-binding capacity, linked to its branched structure, low molecular weight, and high uronic acid/fucose content.
Conclusions:
- Extraction method is a critical factor determining the physicochemical properties and functional activity of Laminaria japonica polysaccharides.
- Acid-assisted extraction offers a promising approach for obtaining LP with enhanced bile acid-binding capacity.
- These findings provide a basis for optimizing LP preparation and evaluating their hypolipidemic effects.
