Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

mRNA Delivery by Lipoamino Fatty Acid-Peptide Polyplexes in Different Lung Cell Models and Lungs.

Polymers·2026
Same author

[Report from the 16th annual meeting of the DFBK, 16-17th January 2026 in Ulm].

Urologie (Heidelberg, Germany)·2026
Same author

A large-scale, multitask, multisensory dataset for climate-aware crop monitoring in the US from 2018-2022.

Scientific data·2026
Same author

Blood-brain barrier opening as a predictor of epilepsy and mortality after subarachnoid haemorrhage.

EBioMedicine·2025
Same author

Single-Cell and Spatial Transcriptomic Profiling of Penile Squamous Cell Carcinoma Reveals Dynamics of Tumor Differentiation and Immune Microenvironment.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Development of a New Ramus Anterior Vertical Reference Line for the Evaluation of Skeletal and Dental Changes as a Decision Aid for the Treatment of Crowding in the Lower Jaw: Extraction vs. Nonextraction.

Journal of clinical medicine·2025

Related Experiment Video

Updated: Feb 24, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

8.7K

Adenovirus Particle Quantification in Cell Lysates Using Light Scattering.

Adrian Hohl1, Anne Sophie Ramms1, Christian Dohmen2

  • 11 Klinikum Rechts der Isar, Technische Universität München , Klinik und Poliklinik für Urologie, München, Germany.

Human Gene Therapy Methods
|August 16, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a new light scattering method for quantifying adenovirus particles in crude cell lysates. This rapid technique enables better monitoring of virus recovery during production and downstream processing.

Keywords:
adenovirusdownstream processinglight scatteringquantification

More Related Videos

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

15.3K
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

16.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

8.7K
An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

15.3K
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

16.5K

Area of Science:

  • Biotechnology
  • Virology
  • Bioprocessing

Background:

  • Adenoviral vector production is crucial for therapeutics.
  • Current methods for adenovirus particle titer measurement require purified samples.
  • Lack of rapid quantification methods for intermediate and crude lysates hinders process optimization.

Purpose of the Study:

  • To develop a rapid and reliable method for quantifying adenovirus particles in non-purified cell lysates.
  • To enable optimization and validation of cell cultures and downstream processing steps.
  • To improve monitoring of virus recovery throughout the production process.

Main Methods:

  • Application of light scattering for virus quantification.
  • Enzymatic treatment of crude lysates with phospholipase A2 and benzonase.
  • Filtration through a 0.22 μm filter cartridge prior to light scattering analysis.

Main Results:

  • A precise method for fast and easy determination of total adenovirus particle numbers in cell lysates was established.
  • Light scattering can be effectively used for quantifying adenovirus particles in crude, non-purified samples.
  • The method allows for monitoring virus recovery across all downstream processing stages.

Conclusions:

  • The developed light scattering method offers a significant advancement for adenovirus production.
  • It provides a crucial tool for real-time process monitoring and optimization.
  • This technique facilitates efficient quality control in adenoviral vector manufacturing.