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Age-associated mRNA expression changes in bovine endometrial cells in vitro
Nao Tanikawa1, Ayaka Ohtsu1, Ryouka Kawahara-Miki2
1Laboratory of Animal Reproduction, Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan.
Reproductive Biology and Endocrinology : RB&E
|August 16, 2017
Summary
Aging impairs endometrial cell function, increasing inflammation and interferon signaling. Aged cells show reduced viability and impaired response to interferon tau (IFNT), impacting fertility.
Area of Science:
- Reproductive Biology
- Cellular Aging
- Immunology
Background:
- Endometrial cell dysfunction is linked to infertility.
- Advanced maternal age reduces fertility due to cellular aging.
- Uterine age-dependent changes are understudied.
Purpose of the Study:
- Investigate age-dependent changes in bovine endometrial cells.
- Examine the effects of Interferon tau (IFNT) on aged endometrial cells.
- Identify molecular pathways affected by aging in the uterus.
Main Methods:
- Next-generation sequencing and real-time PCR analyzed mRNA expression.
- Bovine endometrial cells from young and aged cows were used in vitro.
- IFNT treatment effects were assessed based on animal age.
Main Results:
- Aged endometrial cells showed activated inflammation and interferon-signaling pathways.
- DNA damage checkpoints were activated, and mitotic mechanisms inhibited in aged cells.
- Aged cells had lower viability and a diminished response to IFNT stimulation.
Conclusions:
- Aged endometrial cells display heightened inflammation and impaired cell division.
- High basal interferon-related gene levels in aged cells suggest "inflammaging".
- Age-related uterine changes negatively affect cellular function and response to pregnancy signals.
