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[Rat lymphoid cell lines producing human T cell leukemia virus-I]
[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|January 1, 1987
Summary
Researchers established three rat lymphoid cell lines (TARS-1, TARL-2, TART-1) by cocultivating rat cells with adult T cell leukemia (ATL) cells. These cell lines produce human T cell leukemia virus-I (HTLV-I) and express HTLV-I associated antigens.
Area of Science:
- Immunology
- Virology
- Cell Biology
Context:
- Adult T cell leukemia (ATL) is a malignancy of mature T lymphocytes.
- Human T cell leukemia virus-I (HTLV-I) is the causative agent of ATL.
- Establishing reliable in vitro models is crucial for studying ATL pathogenesis and developing therapies.
Purpose:
- To establish novel rat lymphoid cell lines for studying HTLV-I and ATL.
- To characterize the phenotype and biological properties of the newly derived cell lines.
- To investigate the potential of these cell lines in generating HTLV-I-specific immune responses in vivo.
Summary:
- Cocultivation of rat lymphoid cells with ATL cells yielded three cell lines (TARS-1, TARL-2, TART-1) that produce HTLV-I and express ATL-associated antigens.
- Cytogenetic and surface phenotype analyses confirmed their lymphoid origin, with TARS-1 and TART-1 exhibiting T cell characteristics and expressing rat IL-2 receptors.
- Transplantation studies and induction of HTLV-I carrier rats demonstrated the utility of these cell lines for immunological investigations, including antibody production against HTLV-I specific polypeptides.
Impact:
- The established rat lymphoid cell lines provide valuable tools for investigating HTLV-I biology and ATL.
- These cell lines can be used to study host immune responses to HTLV-I infection.
- The findings contribute to the development of diagnostic and therapeutic strategies for HTLV-I-associated diseases.