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Best practices in cell culture: an overview
John M Baust1, Gertrude Case Buehring2, Lia Campbell3
1CPSI Biotech, 2 Court St., Owego, NY, 13827, USA.
In Vitro Cellular & Developmental Biology. Animal
|August 16, 2017
Summary
This series provides essential best practices for animal cell culture, covering media, serum, cell handling, authentication, contamination control, and preservation. It aims to guide novice and experienced researchers in maintaining high-quality cell cultures.
Area of Science:
- Life Sciences
- Biotechnology
- Cell Biology
Background:
- A significant gap exists in accessible information regarding optimal animal cell culture techniques.
- Entry-level scientists and those needing a refresher require guidance on fundamental cell culture procedures.
Purpose of the Study:
- To address the unmet need for comprehensive best practices in animal cell culture.
- To provide a detailed guide covering critical aspects from media preparation to cell preservation.
Main Methods:
- A series of articles will be published over six months.
- Each article focuses on specific, crucial elements of animal cell culture.
Main Results:
- The series will cover media selection and serum evaluation.
- Best practices for cell receipt, naming, authentication, contamination control, cryopreservation, thawing, storage, and shipping will be detailed.
Conclusions:
- This resource aims to improve the quality and reliability of animal cell culture experiments.
- Adherence to these best practices will enhance reproducibility and reduce experimental errors.
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