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Subcellular Imaging of Neuronal Calcium Handling In Vivo

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Published on: March 17, 2023

Methods to Visualize MAVS Subcellular Localization.

Christine Vazquez¹, Dia C Beachboard¹, Stacy M Horner^2,3

¹Department of Molecular Genetics and Microbiology, Duke University Medical Center, 213 Research Dr., Box 3053, Durham, NC, 27710, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 16, 2017

Summary

This summary is machine-generated.

Mitochondrial antiviral signaling (MAVS) protein is crucial for innate immunity and found in various cell compartments. This study details methods to analyze MAVS protein localization within cells.

Keywords:

Endoplasmic reticulum Fractionation Immunofluorescence Interferon MAM MAVS Mitochondria Peroxisomes

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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

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Published on: February 18, 2014

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Mitochondrial antiviral signaling (MAVS) protein acts as a key adaptor in innate immune responses against viruses.
MAVS localizes to mitochondria, mitochondrial-associated ER membrane (MAM), and peroxisomes to mediate its functions.
Understanding MAVS subcellular localization is essential for studying its role in antiviral immunity.

Purpose of the Study:

To describe protocols for detecting MAVS protein localization within intracellular compartments.
To provide methods for analyzing MAVS distribution in cellular membranes.

Main Methods:

Biochemical fractionation to isolate specific membrane compartments like MAMs, mitochondria, and peroxisomes.
Immunofluorescence assays using antibodies against specific membrane markers to visualize MAVS distribution.

Main Results:

Established protocols for biochemical fractionation and immunofluorescence to detect MAVS localization.
Demonstrated visualization of MAVS distribution across mitochondria, MAM, and peroxisomes.

Conclusions:

Biochemical fractionation and immunofluorescence are effective methods for analyzing MAVS subcellular localization.
Accurate MAVS localization analysis is critical for understanding its function in antiviral innate immunity.