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Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
Published on: February 5, 2018
Handling of Gametes for In Vitro Insemination in Birds
Shusei Mizushima1, Mei Matsuzaki2,3, Tomohiro Sasanami4,5
1Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan.
Abstract:
A characteristic biological property of avian gamete (e.g., extremely large egg and polyspermic fertilization) does not allow the direct observation of sperm-egg interactions in vitro, but recent research advances make it possible to manipulate the gamete in vitro. Here, we describe the techniques for the handling of gametes required for in vitro fertilization assay. In addition, we also introduce the procedures for sperm-perivitelline membrane assay, intracytoplasmic sperm injection, and ex ovo culture.

