Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Stimuli-Activated01:30

Modified-Release Drug Delivery Systems: Stimuli-Activated

51
Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also...
51

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges and Opportunities for Cleavable Linkers Used in Polymer-Drug Conjugates.

Journal of the American Chemical Society·2026
Same author

Age-Dependent and Sex-Dependent Red Nucleus Morphometry: MRI Evidence With Implications for Neurosurgical Planning.

The Journal of craniofacial surgery·2026
Same author

The role of chemokine receptors in leukemia: implications for prognosis and therapeutic strategies.

Molecular biology reports·2026
Same author

Agroseismology and the impact of farming practices on soil hydrodynamics.

Science (New York, N.Y.)·2026
Same author

Comparative Histological Characterization of the Venom Apparatus in Five Iranian Scorpion Species.

Journal of arthropod-borne diseases·2026
Same author

Multiplex PCR-based identification of two sympatric stem borer species, Sesamia cretica and Sesamia nonagrioides (Lepidoptera: Noctuidae).

Journal of insect science (Online)·2025

Related Experiment Video

Updated: Feb 24, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.6K

Dynamic Covalent Hydrogels for Triggered Cell Capture and Release.

Fatemeh Karimi1, Joe Collins1, Daniel E Heath1

  • 1School of Chemical and Biomedical Engineering, Particulate Fluids Processing Centre and ‡Polymer Science Group, Department of Chemical and Biomolecular Engineering, Particulate Fluids Processing Centre, University of Melbourne , Parkville, Melbourne, Victoria 3010, Australia.

Bioconjugate Chemistry
|August 16, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a novel dual-responsive hydrogel surface for reversible cell capture and release. This phenylboronic acid (PBA)-functionalized material offers a nonfouling, reusable platform for biomedical applications like cell diagnostics.

More Related Videos

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.7K
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

7.7K

Related Experiment Videos

Last Updated: Feb 24, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.6K
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.7K
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

7.7K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Polyethylene glycol (PEG) hydrogels are known for their nonfouling properties.
  • Oxime chemistry provides an efficient method for hydrogel functionalization.
  • Phenylboronic acid (PBA) can interact with carbohydrates on cell surfaces.

Purpose of the Study:

  • To develop a dual-responsive hydrogel surface for reversible cell capture and release.
  • To functionalize a PEG-based hydrogel with PBA groups using oxime chemistry.
  • To evaluate the cell capture and release capabilities of the modified hydrogel.

Main Methods:

  • Incorporation of phenylboronic acid (PBA) groups into an oxime-based polyethylene glycol (PEG) hydrogel.
  • Utilizing oxime chemistry for efficient functionalization with 3,5-diformylphenyl boronic acid.
  • Testing cell capture and release using MCF-7, NIH-3T3, and HUVEC cells at varying pH levels.

Main Results:

  • The PBA-functionalized hydrogel demonstrated efficient, reversible cell capture at pH 6.8 via boronic ester formation.
  • Cell detachment was achieved by increasing the pH to 7.8.
  • The capture-and-release process was effective across multiple cell types (MCF-7, NIH-3T3, HUVECs) with high reusability.

Conclusions:

  • A facile method for preparing PBA-functionalized, dual-responsive hydrogel surfaces was established.
  • The developed material enables efficient and reversible cell capture and release.
  • This technology holds promise for applications in cell diagnostics and cell culture.