Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 24, 2026

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.8K

High-throughput Screening for Protein-based Inheritance in S. cerevisiae.

James S Byers1, Daniel F Jarosz2

  • 1Department of Developmental Biology, Stanford University School of Medicine.

Journal of Visualized Experiments : Jove
|August 16, 2017
PubMed
Summary

Protein-based inheritance, or epigenetics, can occur through protein conformation changes, not just DNA. A new method reveals over 1% of yeast proteins can stably transmit traits, challenging previous assumptions about prion rarity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prion-based protein self-assembly tunes mutagenesis to enable rapid adaptation.

Cell·2026
Same author

The Prion-Like Properties of the INO80 Chromatin Remodeler Regulate Metabolic Gene Expression.

bioRxiv : the preprint server for biology·2026
Same author

eSIG-Net: an interaction language model that decodes the protein code of single mutations.

Nature methods·2026
Same author

eSIG-Net: Accurate prediction of single-mutation induced perturbations on protein interactions using a language model.

bioRxiv : the preprint server for biology·2026
Same author

Towards CRISPR-based editing of the mitochondrial genome in yeast.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Protein structure shapes natural genetic variation and <i>de novo</i> adaptation.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Biological information is typically inherited via DNA sequence changes.
  • Inheritance through protein conformation (epigenetics) is considered rare, with prions as a key example.
  • Known prions often exhibit specific sequence biases (N/Q-rich) and amyloid folds, limiting discovery.

Purpose of the Study:

  • To develop a novel method for identifying proteins capable of epigenetic inheritance.
  • To investigate the prevalence of protein-based inheritance beyond known prion characteristics.
  • To establish a high-throughput screening strategy for protein-based inheritance across the yeast proteome.

Main Methods:

  • Developed a mass action-based assay leveraging transient overexpression of proteins.

More Related Videos

Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K
High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.9K

Related Experiment Videos

Last Updated: Feb 24, 2026

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.8K
Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K
High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.9K
  • Assayed the yeast ORFeome (open reading frameome) for proteins inducing self-templating conformations.
  • Analyzed the frequency and stability of protein-templated heritable traits.

    • Main Results:

    • Identified that over 1% of yeast proteins can induce stable, long-lived biological traits.
    • Demonstrated that protein-based inheritance occurs more frequently than genetic mutation.
    • Showcased a method applicable to high-throughput screening and targeted network analysis.

    Conclusions:

    • Protein-based inheritance is more widespread than previously thought, extending beyond classical prion characteristics.
    • The developed method provides a powerful tool to explore the functional landscape of epigenetic inheritance.
    • This approach enables the investigation of protein-based inheritance's role in various biological processes and networks.