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Association Between C1q/TNF-Related Protein-1 Levels in Human Plasma and Epicardial Adipose Tissues and Congestive
Ying Yang1,2, Si Liu2, Rong-Yi Zhang2
1Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
Insights
Levels of C1q and tumour necrosis factor-related protein 1 (CTRP1) were elevated in patients with congestive heart failure (CHF). Higher CTRP1 is linked to worse CHF prognosis and involves IL-6 and aldosterone pathways.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Endocrinology
Background:
- C1q and tumour necrosis factor-related protein 1 (CTRP1) exhibits anti-atherogenic and anti-inflammatory properties.
- Congestive heart failure (CHF) is a complex cardiovascular condition with significant morbidity.
- Understanding novel biomarkers and pathogenic mechanisms in CHF is crucial for improved patient outcomes.
Purpose of the Study:
- To investigate the association between CTRP1 levels in plasma and epicardial adipose tissue (EAT) with CHF.
- To elucidate the molecular mechanisms underlying CTRP1's role in CHF pathogenesis.
Main Methods:
- Plasma and EAT samples collected from CHF patients and controls.
- Quantification of CTRP1, aldosterone synthase (CYP11B2), and mitogen-activated protein kinase levels using ELISA and Western blotting.
- Assessment of CTRP1 and inflammatory cytokine mRNA expression via RT-PCR.
Main Results:
- Elevated CTRP1 levels observed in plasma and EAT of CHF patients compared to controls.
- Higher plasma CTRP1 at admission correlated with a poorer prognosis post-discharge.
- CTRP1 was found to increase IL-6 mRNA levels and modulate CYP11B2 protein expression, influencing aldosterone release via ERK1/2 and Jak-2 pathways.
Conclusions:
- Plasma and EAT CTRP1 levels are increased in CHF patients.
- CTRP1 contributes to CHF pathogenesis by affecting IL-6 levels and aldosterone release.
- CTRP1 may serve as a prognostic marker in CHF.
Background/Aims:
C1q and tumour necrosis factor-related protein 1 (CTRP1) possesses anti-atherogenic and anti-inflammatory effects. This study investigated whether the CTRP1 levels in the plasma and epicardial adipose tissue (EAT) were associated with congestive heart failure (CHF) and to disclose possible molecular mechanisms.
Methods:
Plasma and tissue samples were obtained from subjects with or without CHF. Plasma levels of CTRP1 were measured by ELISA. The mRNA levels of CTRP1 and inflammatory cytokines were detected by RT-PCR. The protein levels of CTRP1, aldosterone synthase (CYP11B2) and mitogen-activated protein kinase were examined by Western blotting.
Results:
The levels of CTRP1 in the plasma and EAT were higher in the CHF patients than those in the controls. There were no differences in the CTRP1 levels in cardiomyocytes between the CHF group and the non-CHF group. An exploratory survival analysis showed that higher CTRP1 values at admission were associated with a worse prognosis after discharge. CTRP1 increased the IL-6 mRNA level in H295R cells. CTRP1 recruited ERK1/2 and Jak-2 for aldosterone release by modulating the CYP11B2 protein level, and brain natriuretic peptide repressed the CTRP1-induced aldosterone release through the JAK2-STAT3 signalling pathways.
Conclusion:
The CTRP1 levels in the plasma and EAT were increased in the CHF patients. CTRP1 is involved in the pathogenesis of CHF by modulating IL-6 levels and aldosterone release.
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