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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Adoptive Transfer of Isolated Bone Marrow Neutrophils
Jimena Tosello Boari1, Eva Acosta Rodríguez1
1Bioquímica Clínica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
Abstract:
Adoptive transfer experiments of specific cell populations are widely used methods to assess the role of the injected population on an ongoing process. In the last years, new and unprecedented roles in the regulation of immune responses have been reported for neutrophils. The following protocol is to be used to isolate neutrophils from bone marrow and to inject them in an appropriate host to test the role of neutrophils during infection, inflammation or other pathological conditions.
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