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[Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro]

Chu-Wen Sun1, Ya-Qin Zhu

  • 1Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.

Shanghai Kou Qiang Yi Xue = Shanghai Journal of Stomatology
|August 18, 2017
PubMed
Abstract

Insights

Ultrasonic PUI, diode laser, and their combination effectively eliminate Enterococcus faecalis biofilms in root canals. However, 3% sodium hypochlorite irrigation demonstrated superior disinfection efficacy in this study.

Area of Science:

  • Endodontics
  • Microbiology
  • Biomedical Engineering

Background:

  • Enterococcus faecalis is a common pathogen in persistent root canal infections.
  • Effective elimination of E. faecalis biofilms is crucial for endodontic treatment success.
  • Novel disinfection methods are continuously explored to improve root canal sterilization.

Purpose of the Study:

  • To compare the efficacy of different methods in eliminating Enterococcus faecalis from root canals.
  • To evaluate ultrasonic PUI, diode laser, and their combination against E. faecalis.
  • To compare these novel methods with saline and sodium hypochlorite irrigation.

Main Methods:

  • Fifty extracted premolars were artificially infected with E. faecalis.
  • Groups were treated with saline, 3% NaClO, PUI, diode laser, or a combination of PUI and diode laser.
  • Bacterial elimination was assessed by colony-forming units (CFUs) after 48 hours.

Main Results:

  • PUI, diode laser, and their combination significantly reduced E. faecalis CFUs compared to saline.
  • No significant difference was observed among PUI, diode laser, and the combination groups.
  • 3% sodium hypochlorite irrigation achieved the highest disinfection efficacy, with near-zero CFUs.

Conclusions:

  • Ultrasonic PUI, diode laser, and their combination show significant potential for eliminating E. faecalis biofilms.
  • 3% sodium hypochlorite remains the most effective irrigant for root canal disinfection in this study.
  • Further research may explore optimizing these emerging technologies for endodontic applications.

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