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Updated: Feb 24, 2026

Engineering Cell-permeable Protein
Published on: December 28, 2009
Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
Wei-Ming Chien1, Yonggang Liu1, Ana A Dinca1
1University Washington School of Medicine, Room 353, 850 Republican Street, Seatle, WA, 98109, USA.
Abstract:
The technique of Cre-mediated DNA recombination at loxP sites has been used widely in manipulation of the genome in cultured cells and in living organisms. Local delivery of Cre recombinase protein tagged with a cell-penetrating (or permeable) peptide (Cre-CPP) has the advantage of additional spatial and temporal control when compared to genetic delivery methods. In this chapter, we describe protocols for injection-based intramuscular delivery of Cre-CPP dissolved in hydrogel to skeletal muscle and by ultrasound-guided injection to cardiac muscle in mice.
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