Hemoglobin transition in the anuran Pelodytes punctatus

In the anuran Pelodytes punctatus the larval hemoglobin produces five electrophoretic bands. In the premetamorphic period, two other bands appear which are typical of the adult. They gradually substitute the larval bands completely as in other anurans. The pathway of the hemoglobin shift indicates that the synthesis of the adult fractions does not depend on the thyroid hormones for its activation, whereas the disappearance of the larval fractions depends on the destruction of the "larval" red blood cell line. As in other species of anurans which are considered primitive, the hemoglobinic change of Pelodytes punctatus starts earlier and develops slower than in other more evolved anurans. In normal developing specimens of Pelodytes punctatus the change in the hemoglobin fractions occurs very slowly and finishes 4 weeks after the metamorphosis, whereas in "hibernated" specimens the rate of change is higher and it ends earlier, probably as a consequence in the retarded larvae, of the increased sensibility of the hemopoietic tissues to the metamorphosis factors.