Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Copper absorption in sheep.

J C Turner, V Shanks, P J Osborn

    Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Multi-site feasibility of a web-based and telephone navigation intervention to connect lung cancer caregivers in community oncology settings with resources: Protocol for the WF-2301CD Caregiver Oncology Needs Evaluation Tool (CONNECT) trial.

    Contemporary clinical trials·2026
    Same author

    Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants.

    Plant cell reports·2019
    Same author

    Characteristics of a photorespiratory mutant of barley (Hordeum vulgare L.) deficient in phosphogly collate phosphatase.

    Photosynthesis research·2014
    Same author

    Characterization of two chlorophyll b-deficient, azide-derived mutants of Hordeum vulgare cv. Maris Mink.

    Photosynthesis research·2014
    Same author

    The value of mutants unable to carry out photorespiration.

    Photosynthesis research·2014
    Same author

    The isolation and characterisation of a catalase-deficient mutant of barley (Hordeum vulgare L.).

    Planta·2013

    Female sheep absorb copper (Cu) in the abomasum, small intestine, and colon, but not the rumen-reticulum or caecum. Copper absorption occurs via simple diffusion, independent of energy or specific transporters.

    Area of Science:

    • Animal Science
    • Gastrointestinal Physiology
    • Mineral Metabolism

    Background:

    • Understanding copper absorption is crucial for animal nutrition and health.
    • Identifying specific gastrointestinal regions responsible for copper uptake is key to optimizing dietary strategies.
    • Previous research has not fully elucidated the absorption mechanisms and regional specificity of copper in ruminants.

    Purpose of the Study:

    • To determine which regions of the gastrointestinal tract in female sheep absorb copper.
    • To investigate the kinetics and characteristics of copper uptake in vitro.
    • To assess the influence of different copper complexes and inhibitors on copper absorption.

    Main Methods:

    • In vivo studies using female sheep to identify copper-absorbing gastrointestinal regions.

    Related Experiment Videos

  • In vitro experiments measuring copper uptake by intestinal tissues.
  • Testing the effects of various copper concentrations, complexed forms (histidine, lysine, glutamine), and metabolic inhibitors (ouabain, rotenone, oligomycin, 2,4-dinitrophenol, fluoride, zinc) on copper uptake.

    • Main Results:

    • Copper absorption was confirmed in the abomasum, small intestine, and colon of sheep.
    • No significant copper absorption was detected in the rumen-reticulum or caecum.
    • In vitro studies revealed a linear relationship between copper concentration and uptake, suggesting simple diffusion kinetics.
    • Copper complexed with amino acids (histidine, lysine, glutamine) was absorbed similarly to ionic copper.
    • Uptake was not inhibited by ouabain, rotenone, oligomycin, 2,4-dinitrophenol, fluoride, or zinc, indicating a non-saturable, passive transport mechanism.

    Conclusions:

    • The abomasum, small intestine, and colon are the primary sites for copper absorption in female sheep.
    • Copper absorption across the gastrointestinal mucosa appears to be a passive process, likely simple diffusion.
    • The form of copper (ionic vs. amino acid complexed) does not significantly alter absorption rates or mechanisms in vitro.
    • The absence of inhibition by metabolic poisons suggests copper transfer is not energy-dependent.