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Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles.

Ji-Wook Kim1,2,3, Daeha Seo4,5, Jung-Uk Lee1,2,3

  • 1Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.

Nature Protocols
|August 18, 2017
PubMed
Summary

This study introduces mechanogenetics, a nanotechnology tool for precise control of cell surface receptors. It allows researchers to study how mechanical forces and spatial cues influence cell signaling at the single-cell level.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Nanotechnology

Background:

  • Understanding cell signaling requires precise control over molecular interactions.
  • Current methods lack the ability to independently manipulate spatial and mechanical cues at the single-cell level.

Purpose of the Study:

  • To develop and describe a nanotechnology-based tool, mechanogenetics, for spatiotemporal control of cell-surface receptors.
  • To enable precise mechanical and spatial perturbation of single molecules in live cells.

Main Methods:

  • Utilizes magnetoplasmonic nanoparticle (MPN) actuators and micromagnetic tweezers (μMT).
  • MPNs are conjugated to DNA oligonucleotides for specific targeting of SNAP-tagged cell-surface receptors.
  • MPN-receptor interactions are controlled by μMT to apply defined forces and spatial cues.

Main Results:

  • Demonstrates stepwise instructions for mechanogenetic control of receptor clustering and mechanical activation.
  • Successfully applied to SNAP-tagged Notch and VE-cadherin in model cell lines.
  • Shows differential control over spatial and mechanical inputs to receptors.

Conclusions:

  • Mechanogenetics provides a novel method for dissecting the contributions of spatial and mechanical signals in cell signaling.
  • This tool facilitates advanced research in cell mechanobiology and signal transduction pathways.